咖啡代谢物分析

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咖啡代谢物分析 Coffee Metabolite Profiling by LC-MS
        Conditions
Column: ACE Excel 1.7 C18-Amide
Dimensions: 100 x 2.1 mm
Part Number: EXL-1712-1002U
Mobile Phase:
        A: 0.01% formic acid in H2O
B: 0.01% formic acid in MeCN

Time(mins)	%B
0.0	3
2.5	10
8.0	100
8.5	3
10.0	3

        Flow Rate: 0.5 mL/min
Detection: Exactive accurate mass MS system ESI in negative ion mode Analytes between m/z 70-800 monitored
Sample: Metabolites from coffee extracted into cold water by vortexing for 20 mins.
Samples filtered prior to injection onto column and modular Accela LCsystem